4c cat 14052 1 ap proteintech Search Results


rabbit anti human cdk6  (Proteintech)
96
Proteintech rabbit anti human cdk6
Rabbit Anti Human Cdk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk6  (Proteintech)
96
Proteintech cdk6
Cdk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cat no 14052 1 ap  (Proteintech)
96
Proteintech cat no 14052 1 ap
Cat No 14052 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
cat no 14052 1 ap - by Bioz Stars, 2026-02
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anti cdk6  (Proteintech)
97
Proteintech anti cdk6
Anti Cdk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cdk6 - by Bioz Stars, 2026-02
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gapdh  (Proteintech)
90
Proteintech gapdh
Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against cyclin-dependent kinase (cdk) 4  (Proteintech)
90
Proteintech primary antibodies against cyclin-dependent kinase (cdk) 4
PCA combined with 5-FU induces the G1 phase cell cycle arrest in liver cancer cells. (A) Flow cytometry was carried out to evaluate the cell cycle distribution in HepG2, Huh7 and Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Western blot analysis was performed to detect the protein expression levels of CDK4 and CDK6 in (B) HepG2, (C) Huh7 and (D) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Reverse transcription- quantitative PCR was performed to detect the mRNA levels of CDK4 and CDK6 in (E) HepG2, (F) Huh7 and (G) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. * P<0.05 and ** P<0.01. PCA, purpurogallin carboxylic acid; 5-FU, 5-fluorouracil; <t>CDK,</t> <t>cyclin-dependent</t> kinase.
Primary Antibodies Against Cyclin Dependent Kinase (Cdk) 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against cyclin-dependent kinase (cdk) 4/product/Proteintech
Average 90 stars, based on 1 article reviews
primary antibodies against cyclin-dependent kinase (cdk) 4 - by Bioz Stars, 2026-02
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mtch2 antibody  (Proteintech)
93
Proteintech mtch2 antibody
Box plots of <t>MTCH2</t> expression levels and Kaplan-Meier curves for OS: (A) GSE7377, (B) GSE54002, (C) GSE45827, (D) TCGA-BRCA, (E) GSE26459; (F) TCGA-BRCA, (G) microarray data of BC patients, (H) the luminal A subtype, (I) the luminal B subtype, (J) the Her2 + subtype, and (K) the basal subtype. Abbreviations: OS, overall survival.
Mtch2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtch2 antibody - by Bioz Stars, 2026-02
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cd47  (Proteintech)
95
Proteintech cd47
Box plots of <t>MTCH2</t> expression levels and Kaplan-Meier curves for OS: (A) GSE7377, (B) GSE54002, (C) GSE45827, (D) TCGA-BRCA, (E) GSE26459; (F) TCGA-BRCA, (G) microarray data of BC patients, (H) the luminal A subtype, (I) the luminal B subtype, (J) the Her2 + subtype, and (K) the basal subtype. Abbreviations: OS, overall survival.
Cd47, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bcl 2  (Proteintech)
93
Proteintech bcl 2
Box plots of <t>MTCH2</t> expression levels and Kaplan-Meier curves for OS: (A) GSE7377, (B) GSE54002, (C) GSE45827, (D) TCGA-BRCA, (E) GSE26459; (F) TCGA-BRCA, (G) microarray data of BC patients, (H) the luminal A subtype, (I) the luminal B subtype, (J) the Her2 + subtype, and (K) the basal subtype. Abbreviations: OS, overall survival.
Bcl 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gpx4  (Proteintech)
96
Proteintech gpx4
Mechanisms of cell death. (A) Intracellular glutathione (GSH) concentration in SGC7901 cells after 24 h treatment with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (B) Malondialdehyde (MDA) levels in SGC7901 cells following 24 h exposure to control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (C) Fe 2 + concentration in SGC7901 cells treated for 24 h with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (D) <t>GPX4</t> protein expression levels in SGC7901 cells after 24 h incubation with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (E) The cell structure of PB, HA@NPs-PB and Ma/HA@NPs-PB treated for 24 h was observed by an electron microscope. The white arrow is the mitochondrial morphology of untreated cells, the yellow arrow is the mitochondrial morphology of treated cells, and the green arrow is the PB-induced autophagy vesicles. The data are expressed as the means ± SD ( n = 3). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.
Gpx4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rabbit  (Proteintech)
98
Proteintech goat anti rabbit
Mechanisms of cell death. (A) Intracellular glutathione (GSH) concentration in SGC7901 cells after 24 h treatment with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (B) Malondialdehyde (MDA) levels in SGC7901 cells following 24 h exposure to control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (C) Fe 2 + concentration in SGC7901 cells treated for 24 h with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (D) <t>GPX4</t> protein expression levels in SGC7901 cells after 24 h incubation with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (E) The cell structure of PB, HA@NPs-PB and Ma/HA@NPs-PB treated for 24 h was observed by an electron microscope. The white arrow is the mitochondrial morphology of untreated cells, the yellow arrow is the mitochondrial morphology of treated cells, and the green arrow is the PB-induced autophagy vesicles. The data are expressed as the means ± SD ( n = 3). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.
Goat Anti Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PCA combined with 5-FU induces the G1 phase cell cycle arrest in liver cancer cells. (A) Flow cytometry was carried out to evaluate the cell cycle distribution in HepG2, Huh7 and Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Western blot analysis was performed to detect the protein expression levels of CDK4 and CDK6 in (B) HepG2, (C) Huh7 and (D) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Reverse transcription- quantitative PCR was performed to detect the mRNA levels of CDK4 and CDK6 in (E) HepG2, (F) Huh7 and (G) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. * P<0.05 and ** P<0.01. PCA, purpurogallin carboxylic acid; 5-FU, 5-fluorouracil; CDK, cyclin-dependent kinase.

Journal: Experimental and Therapeutic Medicine

Article Title: Purpurogallin carboxylic acid exhibits synergistic effects with 5‑fluorouracil on liver cancer cells in vitro by targeting ABCG2

doi: 10.3892/etm.2024.12564

Figure Lengend Snippet: PCA combined with 5-FU induces the G1 phase cell cycle arrest in liver cancer cells. (A) Flow cytometry was carried out to evaluate the cell cycle distribution in HepG2, Huh7 and Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Western blot analysis was performed to detect the protein expression levels of CDK4 and CDK6 in (B) HepG2, (C) Huh7 and (D) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Reverse transcription- quantitative PCR was performed to detect the mRNA levels of CDK4 and CDK6 in (E) HepG2, (F) Huh7 and (G) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. * P<0.05 and ** P<0.01. PCA, purpurogallin carboxylic acid; 5-FU, 5-fluorouracil; CDK, cyclin-dependent kinase.

Article Snippet: Following blocking with 8% skim milk powder in TBS-Tween-20 (0.1%) (TBST) for 2 h at 28 ̊C, the membranes were incubated with primary antibodies against cyclin-dependent kinase (CDK) 4 (1:2,000; cat no. 11026-1-AP), CDK6 (1:1,000; cat no. 14052-1-AP), ABCG2 (1:1,000; cat no. 27286-1-AP) and GAPDH (1:50,000; cat no. 60004-1-Ig) all from Proteintech Group, Inc. for 16 h at 4 ̊C.

Techniques: Flow Cytometry, Western Blot, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction

Box plots of MTCH2 expression levels and Kaplan-Meier curves for OS: (A) GSE7377, (B) GSE54002, (C) GSE45827, (D) TCGA-BRCA, (E) GSE26459; (F) TCGA-BRCA, (G) microarray data of BC patients, (H) the luminal A subtype, (I) the luminal B subtype, (J) the Her2 + subtype, and (K) the basal subtype. Abbreviations: OS, overall survival.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: Box plots of MTCH2 expression levels and Kaplan-Meier curves for OS: (A) GSE7377, (B) GSE54002, (C) GSE45827, (D) TCGA-BRCA, (E) GSE26459; (F) TCGA-BRCA, (G) microarray data of BC patients, (H) the luminal A subtype, (I) the luminal B subtype, (J) the Her2 + subtype, and (K) the basal subtype. Abbreviations: OS, overall survival.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: Expressing, Microarray

MTCH2 expression was upregulated in BC cell lines: (A) qPCR, (B) Western blot and (C) quantitation of Western blot band intensities; It was successfully suppressed and activated by corresponding lentiviral systems: (C) qPCR, (D) Western blot and (F) quantitation of Western blot band intensities.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: MTCH2 expression was upregulated in BC cell lines: (A) qPCR, (B) Western blot and (C) quantitation of Western blot band intensities; It was successfully suppressed and activated by corresponding lentiviral systems: (C) qPCR, (D) Western blot and (F) quantitation of Western blot band intensities.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: Expressing, Western Blot, Quantitation Assay

MTCH2 promotes cellular growth and cycle progression. CCK-8 assay revealed increased cell viability in MTCH2 overexpressing cell lines (A), and suppressed in silenced cells (B); The represented graph of cell cycle analysis by PI staining and flow cytometry (C–E), transition was arrested in silenced cells (F–I). MCM2, PCNA, Cyclin E1 and CDK2 were up-regulated in MTCH2-overexpressing cells and suppressed in MTCH2-silenced lines (J). Abbreviations: CCK-8, cell counting kit-8; PI, propidium iodide.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: MTCH2 promotes cellular growth and cycle progression. CCK-8 assay revealed increased cell viability in MTCH2 overexpressing cell lines (A), and suppressed in silenced cells (B); The represented graph of cell cycle analysis by PI staining and flow cytometry (C–E), transition was arrested in silenced cells (F–I). MCM2, PCNA, Cyclin E1 and CDK2 were up-regulated in MTCH2-overexpressing cells and suppressed in MTCH2-silenced lines (J). Abbreviations: CCK-8, cell counting kit-8; PI, propidium iodide.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: CCK-8 Assay, Cell Cycle Assay, Staining, Flow Cytometry, Cell Counting

Xenografts in nude mice. Compared to the control group on the right side, overexpression of MTCH2 promoted tumor growth (A&C); silencing of MTCH2 suppressed tumor growth (B&D). Volumes of xenograft tumors were up and down regulated by MTCH2 over-expression and silencing, respectively (E&F). Weights were also up and down regulated by MTCH2 over-expression and silencing (G). (H&I) Protein levels of MTCH2 were confirmed by Western blot in over-expression and silenced models. (J) Immunohistochemical for biomarkers of cellular proliferation and cycle. Scale bar, 100 μm.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: Xenografts in nude mice. Compared to the control group on the right side, overexpression of MTCH2 promoted tumor growth (A&C); silencing of MTCH2 suppressed tumor growth (B&D). Volumes of xenograft tumors were up and down regulated by MTCH2 over-expression and silencing, respectively (E&F). Weights were also up and down regulated by MTCH2 over-expression and silencing (G). (H&I) Protein levels of MTCH2 were confirmed by Western blot in over-expression and silenced models. (J) Immunohistochemical for biomarkers of cellular proliferation and cycle. Scale bar, 100 μm.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: Control, Over Expression, Western Blot, Immunohistochemical staining

MTCH2 activates the PI3K/Akt pathway: (A&B) Gene set enrichment analysis; (C&D) Phosphorylation of PI3K and Akt were enhanced with MTCH2 over-expression and inhibited with MTCH2 silencing.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: MTCH2 activates the PI3K/Akt pathway: (A&B) Gene set enrichment analysis; (C&D) Phosphorylation of PI3K and Akt were enhanced with MTCH2 over-expression and inhibited with MTCH2 silencing.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: Phospho-proteomics, Over Expression

(A) CCK-8 assay indicates that the PI3K/Akt pathway activator IGF-1R rescued the anti-proliferative effect of MTCH2 silencing; the significance of differences between groups were evaluated with t -test. (B–E) Represented grapes of cell cycle analysis by PI staining and flow cytometry, (F) Schematic summarizing the connections between MTCH2, PI3K/Akt, IGF-1R and cell cycle regulation. Abbreviations: CCK-8, cell counting kit-8; PI, propidium iodide.

Journal: Heliyon

Article Title: MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer

doi: 10.1016/j.heliyon.2024.e28172

Figure Lengend Snippet: (A) CCK-8 assay indicates that the PI3K/Akt pathway activator IGF-1R rescued the anti-proliferative effect of MTCH2 silencing; the significance of differences between groups were evaluated with t -test. (B–E) Represented grapes of cell cycle analysis by PI staining and flow cytometry, (F) Schematic summarizing the connections between MTCH2, PI3K/Akt, IGF-1R and cell cycle regulation. Abbreviations: CCK-8, cell counting kit-8; PI, propidium iodide.

Article Snippet: The following antibodies were used: MTCH2 Antibody (Proteintech Cat# 16888-1-AP, RRID: AB_2266733 ), PCNA Antibody (Proteintech Cat# 10205-2-AP, RRID: AB_2160330 ), MCM2 Antibody (Proteintech Cat# 10513-1-AP, RRID: AB_2142131 ), CDK1 Antibody (Proteintech Cat# 19532-1-AP, RRID: AB_10638617 ), CDK6 Antibody (Proteintech Cat# 14052-1-AP, RRID: AB_10642144 ), p-AKT Antibody (Proteintech Cat# 66444-1-Ig, RRID: AB_2782958 ), AKT Antibody (Proteintech Cat# 10176-2-AP, RRID: AB_2224574 ), PI3K Antibody (Abcam Cat# ab32089, RRID: AB_777254 ), p-PI3K Antibody (Cell Signaling Technology Cat# 4228, RRID: AB_659940 ) and GAPDH (Proteintech Cat# 10494-1-AP, RRID: AB_2263076 ).

Techniques: CCK-8 Assay, Cell Cycle Assay, Staining, Flow Cytometry, Cell Counting

Mechanisms of cell death. (A) Intracellular glutathione (GSH) concentration in SGC7901 cells after 24 h treatment with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (B) Malondialdehyde (MDA) levels in SGC7901 cells following 24 h exposure to control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (C) Fe 2 + concentration in SGC7901 cells treated for 24 h with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (D) GPX4 protein expression levels in SGC7901 cells after 24 h incubation with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (E) The cell structure of PB, HA@NPs-PB and Ma/HA@NPs-PB treated for 24 h was observed by an electron microscope. The white arrow is the mitochondrial morphology of untreated cells, the yellow arrow is the mitochondrial morphology of treated cells, and the green arrow is the PB-induced autophagy vesicles. The data are expressed as the means ± SD ( n = 3). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.

Journal: ACS Applied Materials & Interfaces

Article Title: Dual-Targeted Biomimetic Nanoparticles for Enhanced Delivery of Polyphyllin B Synergistically Induce Ferroptosis and Immunogenic Cell Death in Gastric Cancer

doi: 10.1021/acsami.5c13198

Figure Lengend Snippet: Mechanisms of cell death. (A) Intracellular glutathione (GSH) concentration in SGC7901 cells after 24 h treatment with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (B) Malondialdehyde (MDA) levels in SGC7901 cells following 24 h exposure to control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (C) Fe 2 + concentration in SGC7901 cells treated for 24 h with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (D) GPX4 protein expression levels in SGC7901 cells after 24 h incubation with control, PB, HA@NPs-PB, and Ma/HA@NPs-PB. (E) The cell structure of PB, HA@NPs-PB and Ma/HA@NPs-PB treated for 24 h was observed by an electron microscope. The white arrow is the mitochondrial morphology of untreated cells, the yellow arrow is the mitochondrial morphology of treated cells, and the green arrow is the PB-induced autophagy vesicles. The data are expressed as the means ± SD ( n = 3). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.

Article Snippet: Then, CDK4 (Cat. 83994-3-RR, Proteintech, China), CDK6 (Cat. 14052-1-AP, Proteintech, China), CyclinD1 (Cat. 26939-1-AP, Proteintech, China), GPX4 (Cat. 30388-1-AP, Proteintech, China), HER2 (Cat. 18299-1-AP, Proteintech, China), CD44 (Cat. 15675-1-AP, Proteintech, China), galectin-3 (Cat. 14979-1-AP, Proteintech, China), CD47 (Cat. 20305-1-AP, Proteintech, China), HMGB1 (Cat. 18256, Abcam, US) and CRT (Cat. 92516, Abcam, US) were incubated overnight at 4 °C.

Techniques: Concentration Assay, Control, Expressing, Incubation, Microscopy

Antitumor growth effects and biosafety assessment of Ma/HA@NPs-PB on nude mice with HGC27 tumors. (A–C) Mice in the four groups were injected with DMSO, PB, HA@NPs-PB or Ma/HA@NPs-PB. The injection was repeated every 3 days. At the end of the experiment (day 21), the tumor morphology (A), tumor weight (B), and tumor volume (C) were determined. (D–I) Blood cells were measured by routine blood tests. (RBC, red blood cell; PLT, platelet; WBC, white blood cell; ALT, alanine aminotransferase; AST, glutamic oxalacetic transaminase; CREA, blood creatinine). (J) Histological staining of the heart, lungs, liver, spleen, and kidneys to assess the biosafety of the Ma/HA@NPs-PB drug delivery system. (K, L) Immunohistochemical staining for GPX4 with subsequent statistical analysis performed at the Ma/HA@NPs-PB drug delivery system. The data are expressed as the means ± SD ( n = 6). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.

Journal: ACS Applied Materials & Interfaces

Article Title: Dual-Targeted Biomimetic Nanoparticles for Enhanced Delivery of Polyphyllin B Synergistically Induce Ferroptosis and Immunogenic Cell Death in Gastric Cancer

doi: 10.1021/acsami.5c13198

Figure Lengend Snippet: Antitumor growth effects and biosafety assessment of Ma/HA@NPs-PB on nude mice with HGC27 tumors. (A–C) Mice in the four groups were injected with DMSO, PB, HA@NPs-PB or Ma/HA@NPs-PB. The injection was repeated every 3 days. At the end of the experiment (day 21), the tumor morphology (A), tumor weight (B), and tumor volume (C) were determined. (D–I) Blood cells were measured by routine blood tests. (RBC, red blood cell; PLT, platelet; WBC, white blood cell; ALT, alanine aminotransferase; AST, glutamic oxalacetic transaminase; CREA, blood creatinine). (J) Histological staining of the heart, lungs, liver, spleen, and kidneys to assess the biosafety of the Ma/HA@NPs-PB drug delivery system. (K, L) Immunohistochemical staining for GPX4 with subsequent statistical analysis performed at the Ma/HA@NPs-PB drug delivery system. The data are expressed as the means ± SD ( n = 6). ns indicates no statistical significance. ** p < 0.01, *** p < 0.005, **** p < 0.001.

Article Snippet: Then, CDK4 (Cat. 83994-3-RR, Proteintech, China), CDK6 (Cat. 14052-1-AP, Proteintech, China), CyclinD1 (Cat. 26939-1-AP, Proteintech, China), GPX4 (Cat. 30388-1-AP, Proteintech, China), HER2 (Cat. 18299-1-AP, Proteintech, China), CD44 (Cat. 15675-1-AP, Proteintech, China), galectin-3 (Cat. 14979-1-AP, Proteintech, China), CD47 (Cat. 20305-1-AP, Proteintech, China), HMGB1 (Cat. 18256, Abcam, US) and CRT (Cat. 92516, Abcam, US) were incubated overnight at 4 °C.

Techniques: Injection, Staining, Immunohistochemical staining